【文献标题】Enhancing methane production of anaerobic sludge digestion by microaeration: Enzyme activity stimulation, semi-continuous reactor validation and microbial community analysis

【作者】Danian Ruana, Zhen Zhou, Hongjian Pang，et.al

【作者单位】上海电力大学（Shanghai University of Electric）

【文献中引用产品】

微生物蛋白酶（Protease）ELISA试剂盒

微生物α葡萄糖苷酶（α-Glu）ELISA试剂盒

【关键词】Micro-aeration pretreatment，Anaerobic digestion，Methane yield，Enzyme activity，Microbial community

【DOI】doi.org/10.1016/j.biortech.2019.121643

【影响因子(IF)】7.08

【出版期刊】《Bioresource Technology》

【产品原文引用】

Sludge property and enzyme activity

Sludge specific resistance to filtrate (SRF) was monitored according to pressure filtration method. Capillary suction time (CST) was determined with a portable CST 304B instrument (Triton, UK) equipped with an 18 mm diameter funnel and Whatman No.7 chromatographygrade paper. The particle size was measured by SALD-2201 Laser Diffraction Particle Size Analyzer (Shimadzu, Japan). The viscosity was measured by a Brookfield Viscometer (model TD2-LVDV-1). Protease and α-Glucosidase activity of sludge liquor were measured for samples collected from microaeration reactors by using the ELISA kit (Shanghai Hengyuan Biotech) according to reported methods (Zheng et al., 2019).

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