【文献标题】Isolation and identification of senescent renal tubular epithelial cells using immunomagnetic beads based on DcR2
【作者】Jia Chen,Ke-Hong Chen,Bi-Qiong Fu,et.al
【作者单位】第三军医大学(Third Military Medical University)
【文献中引用产品】
人白细胞介素6(IL-6)ELISA试剂盒
人转化生长因子β1(TGF-β1)ELISA试剂盒
【关键词】Cell senescence,Renal tubular epithelial cell,Cell sorting,Decoy receptor 2
【DOI】dx.doi.org/10.1016/j.exger.2017.04.008
【影响因子(IF)】5.10
【出版期刊】《Experimental Gerontology》
【产品原文引用】
Cell supernatants were collected from the control group, passage 5,AGEs and H2O2 groups. After the adherence of MACS-sorted DcR2-positive and negative cells, normal medium was added. The cell supernatant of the two groups was collected after 48 h. The levels of IL-6 and TGF-β1 in the supernatant were measured by ELISA kit (Hengyuan,Shanghai, China), according to the manufacturer's instructions.
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